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TSI slant
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TSI slant : ウィキペディア英語版
TSI slant

The Triple Sugar Iron (TSI) test is a microbiological test roughly named for its ability to test microorganism's ability to ferment sugars and to produce hydrogen sulfide.〔(【引用サイトリンク】title=TSI Reactions )〕 It is often used in the selective identification of enteric bacteria including Salmonella and Shigella.
== Composition ==

The TSI slant is a test tube that contains agar, a pH-sensitive dye (phenol red), 1% lactose, 1% sucrose, 0.1% glucose,〔(【引用サイトリンク】title=TSI )〕 as well as sodium thiosulfate and ferrous sulfate or ferrous ammonium sulfate.
All of these ingredients are mixed together and allowed to solidify in the test tube at a slanted angle. The slanted shape of this medium provides an array of surfaces that are either exposed to oxygen-containing air in varying degrees (an ''aerobic'' environment) or not exposed to air (an ''anaerobic'' environment).
TSI agar medium was developed based on Kligler's iron agar, which had been used for the determination of lactose fermentative bacteria, by addition of sucrose to be able to detect sucrose fermentative bacteria also.

抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)
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